The ratio of the content of the extracellular pool of signaling molecules and the activity of intercellular blood contacts in normal and pathological conditions

Objective. Identification of the ratio of the content of signaling molecules extracellular pool and the activity of intercellular blood contacts in practically healthy people and in pathology.

Material and methods. The results of immunological examination of 840 people aged 21–55, 672 women and 168 men living in Arkhangelsk, including 256 practically healthy people at the time of examination and 584 patients with diagnoses of coronary heart disease, hypertension, staphylococcal bronchitis, Crohn’s colitis, autoimmune thyroiditis are presented. The study included the determination of erythrocytes, platelets, neutrophil granulocytes, lymphocytes aggregation, as well as the study of a hemogram, and immunoassay.

Results. In pathological processes, the levels of blood cells aggregation are 2–9 times higher than those in practically healthy individuals. The highest are the levels of the relative content of aggregated erythrocytes and platelets (2–4 times higher) compared to the proportion of aggregated granulocytes and lymphocytes. In the presence of an autoimmune component, considerably more activity of lymphocyte aggregation is recorded, compared to that of granulocytes. An increase in the activity of erythrocyte and platelet aggregation is associated with an increase in the activity of vasoconstriction reactions-vasodilation by endothelin-1 and the nitric oxide cycle, as well as with an increase in serum concentrations of transferrin, sCD71, IgE, CIC and adhesion molecules with a decrease in the concentration of adrenaline. Higher levels of leukocyte aggregation are associated with cortisol, TNF-α, IgE, CIC and free transferrin receptor, as well as adhesion molecules increase in the blood content.

Conclusion. The combination of high level of neutrophil granulocytes and IgE aggregation causes the formation of irreversible aggregates of leukocytes and can be considered as an unfavorable prognostic sign of the pathological process course. The systemic effect of cytokines in their hyperproduction is an extreme measure to normalize the levels of aggregation of cells in the circulating pool and reflects the risk of developing pathological consequences of excessive aggregation.

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