clinmedКлиническая медицинаClinical Medicine (Russian Journal)0023-21492412-1339ООО «Медицинское информационное агентство»10.30629/0023-2149-2024-102-7-536-541clinmed-883Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL INVESTIGATIONSЗначимость полиморфного варианта rs1050450 гена глутатионпероксидазы 1 в структуре факторов риска прогрессирования ишемической болезни сердцаThe significance of the rs1050450 polymorphic variant of the glutathione peroxidase 1 gene in the structure of risk factors for the progression of coronary artery diseasehttps://orcid.org/0000-0001-7361-2161МуслимоваЭ. Ф.MuslimovaE. F.

Муслимова Эльвира Фаритовна — канд. мед. наук, научный сотрудник лаборатории молекулярно-клеточной патологии и генодиагностики

Томск

Elvira F. Muslimova — Candidate of Medical Sciences, Researcher at the Laboratory of Molecular Cell Pathology and Genodiagnostics 

Tomsk

muslimovef@yandex.ruhttps://orcid.org/0000-0003-3667-9599РеброваТ. Ю.RebrovaT. Yu.

Реброва Татьяна Юрьевна — канд. мед. наук, научный сотрудник лаборатории молекулярно-клеточной патологии и генодиагностики

Томск

Tatyana Yu. Rebrova — Candidate of Medical Sciences, Researcher at the Laboratory of Molecular Cell Pathology and Genodiagnostics 

Tomsk

https://orcid.org/0000-0002-8070-2234КужелеваЕ. А.KuzhelevaE. A.

Кужелева Елена Андреевна — канд. мед. наук, старший научный сотрудник отделения патологии миокарда

Томск

Elena A. Kuzheleva — Candidate of Medical Sciences, Senior Researcher at the Department of Myocardial Pathology of the Cardiology Research Institute

Tomsk

https://orcid.org/0000-0002-9488-6900ГарганееваА. А.GarganeevaA. A.

Гарганеева Алла Анатольевна — д-р мед. наук, профессор, заведующая отделением патологии миокарда

Томск

Alla A. Garganeeva — Doctor of Medical Sciences, Professor, Head of the Department of Myocardial Pathology

Tomsk

https://orcid.org/0000-0001-6066-3998АфанасьевС. А.AfanasyevS. A.

Афанасьев Сергей Александрович — д-р мед. наук, профессор, заведующий лаборатории молекулярно-клеточной патологии и генодиагностики

Томск

Sergey A. Afanasyev — Doctor of Medical Sciences, Professor, Head of the Laboratory of Molecular Cell Pathology and Genodiagnostics 

Tomsk

Научно-исследовательский институт кардиологии, Томский национальный исследовательский медицинский центр Российской академии наукРоссияTomsk National Research Medical Center of the Russian Academy of SciencesRussian Federation2024211120241027536541Copyright © Муслимова Э.Ф., Реброва Т.Ю., Кужелева Е.А., Гарганеева А.А., Афанасьев С.А., 20242024Муслимова Э.Ф., Реброва Т.Ю., Кужелева Е.А., Гарганеева А.А., Афанасьев С.А.Muslimova E.F., Rebrova T.Y., Kuzheleva E.A., Garganeeva A.A., Afanasyev S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.clinmedjournal.com/jour/article/view/883

Глутатионпероксидаза 1 (GPX1) играет важнейшую роль в блокировании участия окислительного стресса в формировании атеросклеротических бляшек. Известен полиморфный вариант rs1050450, который может быть связан с активностью фермента и тем самым влиять на прогрессирование ишемической болезни сердца (ИБС).

Цель

Цель. Оценить связь варианта rs1050450 гена GPX1 с факторами риска прогрессирования ИБС: нарушениями углеводного обмена и концентрацией атерогенных фракций липопротеинов.

Материал и методы

Материал и методы. В исследование вошли 168 пациентов с ИБС, из них 54,8% имели в анамнезе инфаркт миокарда (ИМ). Сахарный диабет выявлен у 29,8%, нарушение толерантности к глюкозе (НТГ) — у 10,1% пациентов. Определен вариант rs1050450 (Pro200Leu, 599C > T) гена GPX1 с использованием TaqMan-зондов.

Результаты

Результаты. Гомозиготами CC были 141 пациент, гетерозиготами CT — 27 пациентов. Гомозиготы TT в выборке отсутствовали. Среди гетерозигот частота ИМ оказалась ниже, чем среди гомозигот CC — 37% против 58,2% (p = 0,043). У гетерозигот уровни общего холестерола и липопротеинов низкой плотности (ЛПНП) были ниже, чем у гомозигот: 3,8 (3,4; 4,5) против 4,3 (3,7;5,6) ммоль/л (p = 0,047) и 1,9 (1,4; 2,3) против 2,5 (1,9;3,3) ммоль/л (p = 0,005) соответственно. В то же время среди гетерозигот оказалась выше частота НТГ (22,2% против 7,8%, p = 0,023).

Заключение

Заключение. Среди больных ИБС носители генотипа CT варианта rs1050450 отличались меньшей частотой ИМ, но большей встречаемостью нарушений углеводного обмена. Гетерозиготы имели меньший уровень атерогенных ЛПНП и общего холестерина.

Glutathione peroxidase 1 (GPX1) plays a crucial role in blocking the involvement of oxidative stress in the formation of atherosclerotic plaques. A polymorphic variant rs1050450 is known, which may be associated with enzyme activity and thereby affect the progression of ischemic heart disease (IHD).

Objective

Objective. To assess the association of the rs1050450 variant of the GPX1 gene with risk factors for the progression of IHD: carbohydrate metabolism disorders and the concentration of atherogenic lipoprotein fractions.

Material and methods

Material and methods. The study included 168 patients with IHD, of whom 54.8% had a history of myocardial infarction (MI). Diabetes mellitus was identified in 29.8%, and glucose intolerance (GT) in 10.1% of patients. The rs1050450 variant (Pro200Leu, 599C > T) of the GPX1 gene was determined using TaqMan probes.

Results

Results. There were 141 patients with CC homozygotes and 27 patients with CT heterozygotes. TT homozygotes were absent in the sample. Among heterozygotes, the frequency of MI was lower than among CC homozygotes—37% vs. 58.2% (p = 0.043). Heterozygotes had lower levels of total cholesterol and low-density lipoproteins (LDL) compared to homozygotes: 3.8 (3.4; 4.5) vs. 4.3 (3.7; 5.6) mmol/L (p = 0.047) and 1.9 (1.4; 2.3) vs. 2.5 (1.9; 3.3) mmol/L (p = 0.005), respectively. At the same time, the frequency of GT was higher among heterozygotes (22.2% vs. 7.8%, p = 0.023).

Conclusion

Conclusion. Among patients with IHD, carriers of the CT genotype of the rs1050450 variant showed a lower frequency of MI but a higher incidence of carbohydrate metabolism disorders. Heterozygotes had lower levels of atherogenic LDL and total cholesterol.

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The authors declare that there are no conflicts of interest present.